See the phenotype
Use high-content cell painting imaging to capture treatment-associated changes in cell appearance and organization.
High-content morphology
New BioSpyder assay
TempO-Paint™
See the phenotype
Read the mechanism
Pair cell painting morphology with TempO-Seq® gene expression profiling from the same biological wells, connecting high-content imaging and transcriptomics in one experimental context.
No replicate plates required for paired readouts
01 / cell paintingCell morphology
The same biological wells
02 / TempO-SeqGene expressionComplementary molecular readout
96- or 384-wellCell painting plate formats
Same-wellLysis after imaging, without separate assay plates
FlexibleFocused gene panels to whole transcriptome
A connected view of biology
One experimental context
One experimental context
Two complementary readouts
Cell painting reveals how a treatment changes cellular morphology. TempO-Seq adds a gene expression view of the response. TempO-Paint™ brings those measurements together using material from the same wells so phenotypic observations can guide mechanistic follow-up.
Read the mechanism
Profile gene expression from the cell painting-treated wells using the extraction-free TempO-Seq platform.
Transcriptomic responseSame-well integration
Collect both molecular and image-based readouts without needing a separate replicate plate for the pair.
Connected interpretation
Interpret morphology alongside gene expression signatures within the same treatment context.
Confident prioritization
Use complementary evidence to select compounds and conditions for deeper investigation.
The assay workflow
TempO-Paint™ workflow
Cell painting imaging and TempO-Seq transcriptomics from the same biological wells
01
Cell painting
Treat, fix, stain and image cells in a 96- or 384-well plate
Cell painting images
02
Same-well lysis
Add lysis buffer directly to the cell painting-treated wells
Lyse cells in the same wells
03
TempO-Paint™ assay
Use the same-well lysate with TempO-Seq chemistry
04
Sequencing analysis
Generate gene expression profiles and dose-response results
Example dose-response results
Concentration-dependent gene expression
Dual phenotypic and molecular insights from the same biological wells — no replicate plates required
From observation to insight
Connect phenotype to mechanism
Following high-content imaging, TempO-Seq profiling provides complementary molecular data for interpreting how cells respond to a treatment.
- Explore concentration-dependent expression patterns
- Connect cellular changes with transcriptional responses
- Prioritize compounds, doses and follow-up experiments
Example dose-response visualizationGene expression vs concentration
Built on TempO-Seq®
Established gene expression chemistry, connected to morphology
TempO-Paint™ builds on the TempO-Seq platform to combine cell painting with transcriptomic analysis. TempO-Seq uses templated detector oligo chemistry to read gene expression from lysates, without RNA extraction or cDNA synthesis.
Streamlined preparation
Extraction-free TempO-Seq processing simplifies the molecular workflow, allowing the cell painting lysate to be used directly in the assay.
Flexible assay content
Assay content can range from focused, customizable gene panels to whole-transcriptome coverage, depending on study goals.
High-throughput potential
Sample tagging and multiplexed sequencing support efficient processing of many samples in a single sequencing run.
Research applications
Where TempO-Paint™ fits
Designed for research teams that want to place high-content image features and gene expression signatures in a shared experimental context.
Cell painting plates
Add transcriptomic information to a cell painting workflow using the same biological wells.
Cell-culture screens
Examine cellular morphology and molecular response across screened treatments or conditions.
Dose-response studies
Explore how changes in concentration relate to transcriptomic and phenotypic readouts.
Mechanistic follow-up
Investigate gene expression patterns underlying phenotypic observations of interest.
Compound prioritization
Use connected molecular and imaging evidence to help identify the most informative follow-up candidates.
Organoid screens
Connect image-based phenotypes with gene expression in organoid screening studies, with assay setup tailored to the model.
Not sure which gene panel fits your screening objective?Ask about assay design →
Common questions
TempO-Paint™
TempO-Paint™
FAQ
Answers to the questions most likely to come up when planning a paired cell painting and gene expression study.
Have a different question? Contact us →What is TempO-Paint™?
TempO-Paint™ combines high-content cell painting imaging and TempO-Seq gene expression profiling in the same experimental context. Cells are imaged first, then lysed in the same wells for molecular analysis.
Do I need a second plate for transcriptomics?
No separate replicate plate is required to obtain the paired cell painting and TempO-Seq readouts. This does not remove the need for appropriate biological replicates, controls or study-specific validation.
What plate format does the workflow use?
The provided TempO-Paint™ workflow supports cell painting and same-well lysis in 96- or 384-well formats. The illustrated example shows a 384-well plate; contact BioSpyder to confirm the setup for your study.
Can I use a focused gene panel or whole transcriptome?
TempO-Seq supports assay content ranging from customizable, focused gene panels to whole-transcriptome profiling. BioSpyder can help identify appropriate content for the biology and research questions you want to investigate.
Does the assay require RNA extraction?
The TempO-Seq chemistry described for TempO-Paint™ is extraction-free and uses the lysate from the cell painting-treated wells without an RNA extraction or cDNA synthesis step.
Does TempO-Paint™ replace cell painting?
No. Cell painting remains the imaging readout. TempO-Paint™ complements those image-based measurements with gene expression data from the same wells to support phenotype-to-mechanism investigations.
How do I plan a TempO-Paint™ study?
Email info@biospyder.com with your cell model, planned cell painting workflow, treatment layout and gene expression goals. The BioSpyder team can discuss suitable assay content and project requirements.
Connect phenotype to mechanism
Get more out of every cell painting experiment
Explore a same-well workflow that brings morphology and transcriptomics together. Tell us about your study and we will help you consider the next steps.
Contact BioSpyder
info@biospyder.com
