Sequencing savings with TempO‑Seq

Fewer reads per sample changes the entire cost equation

The most important sequencing advantage of TempO‑Seq is read depth. The S1500+ panel can be planned from about 0.67 million reads per sample, while TempO‑Seq Whole Transcriptome is typically planned at about 3–6 million reads per sample. Standard RNA‑Seq projects are commonly planned at 20–50 million reads per sample. That difference determines how many flow cells you need and how much sequencing costs.

Compare the workflows Request a project quote

Read depth first

Start with how many reads the biology actually needs

Rather than centering the page on a single sequencer, this version starts with the assay. Once the read requirement is set, the number of flow cells and the sequencing spend fall out of the math automatically.

0.67M

S1500+ panel

The lowest read-depth option on this page. At 96 samples, the project needs about 64.3 million reads before any planning buffer, which can fit comfortably on a small Illumina flow cell.

3–6M

Whole Transcriptome

Even the broad TempO‑Seq Whole Transcriptome workflow is planned at a fraction of the reads commonly used for standard RNA‑Seq. The calculator lets visitors move anywhere within the 3–6 million range.

20–50M

Standard RNA‑Seq

This is where the cost gap opens. More reads per sample mean more flow cells, and standard RNA‑Seq also adds library preparation plus RNA purification before sequencing begins.

Interactive side-by-side calculator

Toggle the panel, read depth and Illumina flow cell

This calculator mirrors the argument in your email: compare the lower read requirement of TempO‑Seq with a conventional Illumina RNA‑Seq workflow that also carries RNA library-prep and purification costs. Choose among several smaller Illumina flow-cell configurations to see how the economics change.

TempO‑Seq panel

S1500+ is fixed at 0.67M reads/sample. Whole Transcriptome unlocks a 3–6M slider.

Illumina flow-cell family

Each choice uses the shorter-cycle reagent for TempO‑Seq and the paired longer-cycle reagent for the RNA‑Seq comparison.

Illumina RNA‑Seq library prep

Uses the 96-sample kit-cost examples from the email context you supplied and scales them by sample count.

Standard RNA‑Seq reads per sample

The email comparison starts at 20M reads/sample and notes 20M–50M as the conventional range.

Sample count

Project size96 samples

TempO‑Seq

S1500+ sequencing

NextSeq 500/550 High Output, 75 cycles

Reads per sample0.67M

Total reads needed64.3M

Flow cells required1

Illumina flow-cell list price$1,899

RNA purification$0

Sequencing spend$1,899

Standard Illumina RNA‑Seq

Stranded mRNA workflow

NextSeq 500/550 High Output, 150 cycles

Reads per sample20M

Total reads needed1.92B

Flow cells required5

Illumina flow-cell list price$3,638

RNA library-prep cost$3,446.80

RNA purification at $15/sample$1,440

Sequencing spend$18,190

Prep + purification + sequencing$23,076.80

Read-depth reduction29.9×

Flow cells avoided4

Sequencing spend difference$16,291

RNA‑Seq prep + sequencing burden$240.38/sample

Planning illustration only. The TempO‑Seq side compares sequencing burden and does not include the TempO‑Seq assay-kit quote. The RNA‑Seq side intentionally includes library prep plus $15/sample RNA purification because those are additional conventional workflow costs highlighted in your email. Flow-cell pricing uses a public Illumina US list-price snapshot revised May 31, 2024. Actual pricing, discounts, read structure, utilization, output and project design may differ.

The 96-sample argument

The email logic becomes very easy to see

With the default 96-sample comparison, a 400 million read flow cell makes the point immediately. The TempO‑Seq project fits on one flow cell, while a 20 million read RNA‑Seq project needs five. The conventional workflow then adds RNA library prep and purification on top.

96 samples with S1500+

At 0.67 million reads per sample, 96 samples require approximately 64.3 million reads. That is well below the 400 million read capacity used in the original NextSeq example.

96samples

0.67Mreads per sample

64.3Mtotal reads

96 samples with standard RNA‑Seq

At just 20 million reads per sample, the same 96 samples require 1.92 billion reads. Dividing that by a 400 million read flow-cell capacity gives 4.8, which means five flow cells before considering any utilization buffer.

96samples

20Mreads per sample

5400M flow cells

Illumina flow-cell price references

Multiple public list-price comparisons, not one cherry-picked platform

The calculator includes several Illumina flow-cell families so a visitor can test the same read-depth argument across different throughput levels. The shorter-cycle and longer-cycle prices below are paired within the same platform family to reflect the cost logic in your email.

Illumina platform / flow cell	Published read capacity used	Shorter-cycle reagent used for TempO‑Seq comparison	Public list price	Longer-cycle reagent used for RNA‑Seq comparison	Public list price
NextSeq 500/550 High Output	Up to 400M reads	75 cycles	$1,899	150 cycles	$3,638
NextSeq 1000/2000 P2	Up to 400M reads	100 cycles	$1,455	200 cycles	$2,736
NextSeq 2000 P3	Up to 1.1B reads in the list-price snapshot	100 cycles	$3,331	200 cycles	$4,612
NovaSeq 6000 SP	650M reads at the low end of Illumina's published range	100 cycles	$2,417	200 cycles	$3,166

Price source: Illumina public US list-price catalogue, revision dated May 31, 2024. Capacity source: Illumina product/specification pages. The public Illumina store currently may require sign-in to display live account pricing; therefore this page labels the figures as public list-price reference values rather than implying a live customer quote.

Illumina public list-price catalogue ↗ NextSeq 1000/2000 reagents ↗ NovaSeq 6000 specifications ↗ Illumina Stranded mRNA Prep ↗

RNA‑Seq cost layers

Sequencing is only part of the conventional RNA‑Seq bill

The email argument is strongest when the page shows all three conventional cost layers together: library prep, RNA purification and sequencing. The 96-sample example below keeps those costs visible instead of comparing flow-cell prices in isolation.

Workflow	96-sample library-prep example	RNA purification	20M reads/sample on 400M flow cells	Flow cells required	Why it matters
Illumina Stranded mRNA comparison	$3,446.80	$1,440	1.92B total reads	5	The cheaper RNA library-prep example still carries purification and five 400M sequencing runs at 20M reads/sample.
Illumina Stranded Total RNA comparison	$7,364.72	$1,440	1.92B total reads	5	The higher library-prep spend makes the total cost gap even larger before sequencing depth increases to 30M or 50M.

The two library-prep values above are the 96-sample example costs supplied in your BioSpyder email context. The calculator scales them proportionally for illustration. They are not represented as live Illumina account pricing.

Use fewer reads to make more samples fit

Tell BioSpyder the panel, sample count and study goals. We can help translate the read requirement into a practical sequencing plan and compare it with a conventional RNA‑Seq workflow.

Request sequencing

Home
Technology

BioSpyder Technology
Biology to Barcodes™

Explore TempO-Seq chemistry, workflow, publications, resources, and platform advantages.
View Technology

Technology Overview
TempO-Seq Introduction Biology to Barcodes™ TempO-Seq Workflow TempO-SPan

Technical Resources
Resources Publications Detector Oligo Design Limitations of RNA-Seq Patents
Products

BioSpyder Products
TempO-Seq Assays & Services

Browse BioSpyder platforms, sample types, panels, analysis tools, and service offerings.
View Products

Platforms
TempO-Seq TempO-LINC TempO-Seq miRNA TempO-SNP

Sample Types
Cell lysates and Purified RNA FFPE Dried Blood Spot Other Sample Types

Panels & Data
Panels TempO-Seq Portal Data Analysis Bioinformatics Services

Partners & Programs
Our Distributors Collaborations Request Information
News
About Us

About BioSpyder
Company, Team & Global Reach

Learn about BioSpyder leadership, distributor coverage, grants, contracts, and upcoming tradeshows.
Meet the Team

Company
Team Our Distributors Grants & Contracts

Events & Policies
Tradeshows Financial Conflict of Interest Contact BioSpyder
FAQ's
Customer Page
Contact