01 / AFFORDABLE
Make sequencing capacity go further
Pool large numbers of indexed samples while sequencing short target-identifying barcodes.
Biology to Barcodes™
Gene expression
built for
scale.
TempO-Seq™ brings high-throughput targeted sequencing to studies that need to measure hundreds to thousands of genes across hundreds to thousands of samples — without the complexity of conventional RNA-Seq.
No RNA extraction required
Up to 6,144 samples per run
Whole transcriptome or custom panels
10 pg
Low-input capability
6,144
Samples in one run
<1 hour
Hands-on time
1 day
Assay workflow
TempO-Seq
Measure more biology. Process more samples.
TempO-Seq uses highly specific detector oligos and sequencing-based barcodes to simplify gene-expression profiling. Flexible assay content, short barcode reads and streamlined sample preparation make large studies practical without sacrificing biological depth.
New developments
Expanding what TempO-Seq can measure.
BioSpyder is extending the TempO-Seq platform into new biological resolutions and RNA classes while maintaining the same focus on scalable, practical workflows.
Applications
Built for biology that needs throughput.
Toxicology
Making chemical safety better
Bring molecular endpoints into high-throughput screening, concentration-response studies and next-generation risk-assessment workflows.
Explore toxicology
→
Pharma
Make earlier decisions with more biology
Profile compound response at scale to support screening, mechanism-of-action studies, safety assessment and biomarker development.
Explore applications
→
Oncology
Extract more information from precious tissue
Investigate tumor biology, biomarkers and gene-expression signatures using scalable assays compatible with challenging samples including FFPE.
Explore applications
→
Computational biology
Turn large datasets into biological insight
Combine scalable transcriptomic measurements with signatures, machine-learning approaches and computational analysis for classification and prediction.
Explore data analysis
→
Biology to Barcodes™
Simultaneously analyze thousands of targets.
Detector oligos hybridize directly to target RNA sequences. Unbound detectors are removed, correctly paired detectors are ligated, and sample-tagged primers convert those molecular events into sequencing-ready barcodes.
01
Hybridize target-specific detector oligos
02
Remove excess unbound detectors
03
Ligate correctly hybridized detector pairs
04
Amplify, barcode, pool and sequence
Why TempO-Seq
High content without high complexity.
TempO-Seq was designed around the realities of large studies: many samples, flexible biology, limited material and the need to get from experiment to interpretable data quickly.
02 / FLEXIBLE
Work with diverse inputs
Support cell lysates, purified RNA, FFPE and other sample formats across a range of study designs.
03 / SIMPLE
Remove unnecessary workflow
Eliminate multiple conventional RNA-sequencing preparation steps and keep the assay straightforward.
04 / FAST
Get to data sooner
A streamlined one-day assay reduces hands-on time and shortens the path from samples to sequencing.
05 / DEEPER INSIGHT
Measure more of the biology
Interrogate hundreds to thousands of targets rather than relying on a small number of conventional endpoints.
06 / MODULAR
Design around your study
Use whole-transcriptome, surrogate or custom assay content tailored to the targets and species that matter.
Proven at scale
Built for high-throughput biology.
BioSpyder's platform has been deployed across large-scale transcriptomic programs, screening studies and research applications where sample throughput is essential.
Frequently asked
Starting a TempO-Seq study?
Contact BioSpyder with your sample type,
approximate sample count and assay requirements.
The team can recommend the appropriate TempO-Seq
assay or service configuration.
TempO-Seq can support multiple sample formats
including cell lysates, purified RNA, FFPE tissue,
whole blood, dried blood spots and additional
application-specific inputs.
Yes. TempO-Seq assay content can be customized
around specific targets and species in addition
to BioSpyder's existing whole-transcriptome and
surrogate panels.
TempO-Seq does not require proprietary laboratory
instrumentation for the assay workflow and
produces libraries for next-generation sequencing.
Start your study
Bring molecular endpoints to your next experiment.
Tell us your sample type, study size and biological question. BioSpyder can help identify the TempO-Seq workflow and assay content that fits your project.
Contact BioSpyder →