BioSpyder Technologies Inc. has developed a novel product for targeted sequencing called TempO-Seq™, a gene expression profiling tool designed to monitor hundreds to thousands of genes at once in high throughput. TempO-Seq can analyze expression in samples with thousands of cells or from single cells without pre-amplification, maximizing utilization of precious or limited samples. Sample barcoding, together with sequencing short fragments of each gene, enables pooling up to 6,144 samples in one sequencing run. Assay content is flexible and customizable delivering unprecedented accuracy and sensitivity with simplified data analysis that eliminates the need for bioinformatics. TempO-Seq assays deliver an easy to use solution for customers doing expression profiling for any species.

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Contact us for more information on how to implement molecular endpoints to your high-throughput studies.

Simultaneously analyze thousands of targets

TempO-Seq turns Biology to Barcodes™, bringing the power of targeted sequencing to high-throughput sample processing with the use of template-dependent oligo ligation. The process is both simple and ingenious. Detector oligos bind to specific RNA sequences and unbound oligos are eliminated. After the detectors are ligated and amplified with sample-tagged primers, the library is pooled and sequenced.

 

New developments

 

Covering the biological universe

 
 

Applications

Bring whole transcriptome profiling to your lab.
TempO-Seq opens new worlds of science to almost any genomics lab. Suddenly large scale studies are both affordable and practical, thanks to high target content, high sample throughput, and simple data analysis.

 

Toxicology

Making chemical safety better

Toxicology is one of life science’s most demanding frontiers, where subtle exposures can drive big biological changes and traditional endpoints don’t always tell the full story. TempO-Seq reveals how cells and tissues respond at the gene expression level, delivering clearer mechanistic insight and faster, more confident safety decisions across discovery, development, and regulatory workflows.

 

Pharma

Making it easier to do drug development

Early compound screening is one of pharma’s highest-leverage decision points, where subtle biological signals can determine whether a candidate moves forward or becomes a costly late-stage failure. TempO-Seq delivers high-throughput, gene expression profiling to show how compounds impact biology early, enabling faster, more confident safety and go/no-go decisions.

 

Oncology

Helping in the fight against cancer

Oncology research is one of biomedicine’s most challenging arenas, where tumors evolve quickly and responses can vary dramatically from patient to patient. TempO-Seq reveals the gene expression programs driving cancer behavior and treatment response, enabling clearer biomarker discovery, mechanism insight, and more confident decisions across research and translational studies.

 

Computational Biology

Simplifying data analysis

Computational biology is transforming how we interpret high-dimensional screening data, turning raw transcriptomic profiles into actionable predictions. By combining TempO-Seq’s scalable gene expression readouts with machine-learning models, researchers can classify biological responses, predict molecular initiating events, and uncover mechanistic patterns that traditional endpoints may miss. This enables faster prioritization, better hazard prediction, and more confident decision-making from early screening through advanced research workflows.

 
 

SPECIFICATIONS

 

MORE AFFORDABLE

pooling samples and multiplexing thousands of genes makes sequencing extremely inexpensive

Sequence up to 6,144 samples in one run

FLEXIBLE INPUTS

cell lysates, FFPE or frozen tissue, low input (down to 10pg)

DNA,RNA, protein and more

SIMPLE

no DNA conversion: cell lysates, purified RNA, or frozen/FFPE tissue can go directly in the assay

robust protocol for all sample types

FASTER

one day assay with <1 h hands-on time

simple, streamlined bioinformatics analysis

DEEPER INSIGHTS

analyze thousands of targets while processing hundreds to millions of samples add or subtract targets without any impact on data

MODULAR AND SCALEABLE

whole transcriptome panels, S1500+ surrogate panels, custom panels: add or subtract gene targets

no impact to other targets in your signature

 

FREQUENTLY ASKED QUESTIONS

  • Please email us at info@biospyder.com

  • We typically complete a service project in 4-6 weeks after receipt of samples

  • TempO-Seq can be run on cell lysates, isolated RNA, whole blood, blood spotted on filter paper, and FFPE tissue slides, and others. Please contact us to learn more about what samples we can accept.

For more frequently asked questions, visit our FAQ page.

 

Read more about the impact of TempO-Seq